E-ISSN 2090-0503 | ISSN 1687-7497
 
2023, Vol: 19, Issue: 2
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Original Research 


Antibacterial activity of Chaga mushroom (Inonotus obliquus) extract against Staphylococcus aureus and Salmonella typhimurium: An In Vitro Study

Majdah Mohamed Al-Tuwaijri, Sayed M. S. Abo El-Souad.

Abstract
Inonotus obliquus (Chaga mushroom) is a herbal and edible mushroom widely distributed in different regions, especially temperate regions. Chaga parasites on the trunk of angiosperms and grows, forming an irregular black mass. The medical importance of Chaga has been reported from ancient cultures to data due to its activity against a wide range of diseases. In the present study, the ethyl acetate extract of the Chaga mushroom has been tested against two bacterial pathogens, Staphylococcus aureus ATCC25923 and Salmonella typhimurium ATCC14028. The antimicrobial activity of the extract showed high activity with inhibition zone diameters of 25 mm for S.aureus and 28 mm for S.typhimurium and minimum inhibitory concentration (MICs) of 3.125 µg/ml for both bacterial pathogens. GC-MS analysis of the extract revealed several compounds with seven major compounds; they are 3-pentanone, propyl butyrate, Ethyl propionate, Trimethylsilymethanol, Neopentyl glycol, n-butyl acetate, and 1-butanol-3-methyl acetate. The antibacterial mode of action of Chaga extract was also elucidated by studying the effect of the extract on cell membrane permeability and recoded high effect on bacterial cell membrane permeability, which was confirmed by transmission electron microscope (TEM) images that revealed leakage of bacterial cell content outside the cells and death.

Key words: Inonotus obliquus extract, Antibacterial activity, Staphylococcus aureus, Salmonella typhimurium, GC-MS, Cell membrane permeability, TEM.


 
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How to Cite this Article
Pubmed Style

Al-Tuwaijri MM, El-Souad SMSA. Antibacterial activity of Chaga mushroom (Inonotus obliquus) extract against Staphylococcus aureus and Salmonella typhimurium: An In Vitro Study. Egypt. J. Exp. Biol. (Bot.). 2023; 19(2): 167-177. doi:10.5455/egyjebb.20230921012834

Web Style

Al-Tuwaijri MM, El-Souad SMSA. Antibacterial activity of Chaga mushroom (Inonotus obliquus) extract against Staphylococcus aureus and Salmonella typhimurium: An In Vitro Study. https://www.egyseb.net//?mno=170350 [Access: December 15, 2023]. doi:10.5455/egyjebb.20230921012834

AMA (American Medical Association) Style

Al-Tuwaijri MM, El-Souad SMSA. Antibacterial activity of Chaga mushroom (Inonotus obliquus) extract against Staphylococcus aureus and Salmonella typhimurium: An In Vitro Study. Egypt. J. Exp. Biol. (Bot.). 2023; 19(2): 167-177. doi:10.5455/egyjebb.20230921012834


Vancouver/ICMJE Style

Al-Tuwaijri MM, El-Souad SMSA. Antibacterial activity of Chaga mushroom (Inonotus obliquus) extract against Staphylococcus aureus and Salmonella typhimurium: An In Vitro Study. Egypt. J. Exp. Biol. (Bot.). (2023), [cited December 15, 2023]; 19(2): 167-177. doi:10.5455/egyjebb.20230921012834


Harvard Style

Al-Tuwaijri, M. M. & El-Souad, . S. M. S. A. (2023) Antibacterial activity of Chaga mushroom (Inonotus obliquus) extract against Staphylococcus aureus and Salmonella typhimurium: An In Vitro Study. Egypt. J. Exp. Biol. (Bot.), 19 (2), 167-177. doi:10.5455/egyjebb.20230921012834


Turabian Style

Al-Tuwaijri, Majdah Mohamed, and Sayed M. S. Abo El-Souad. 2023. Antibacterial activity of Chaga mushroom (Inonotus obliquus) extract against Staphylococcus aureus and Salmonella typhimurium: An In Vitro Study. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 19 (2), 167-177. doi:10.5455/egyjebb.20230921012834


Chicago Style

Al-Tuwaijri, Majdah Mohamed, and Sayed M. S. Abo El-Souad. "Antibacterial activity of Chaga mushroom (Inonotus obliquus) extract against Staphylococcus aureus and Salmonella typhimurium: An In Vitro Study." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 19 (2023), 167-177. doi:10.5455/egyjebb.20230921012834


MLA (The Modern Language Association) Style

Al-Tuwaijri, Majdah Mohamed, and Sayed M. S. Abo El-Souad. "Antibacterial activity of Chaga mushroom (Inonotus obliquus) extract against Staphylococcus aureus and Salmonella typhimurium: An In Vitro Study." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 19.2 (2023), 167-177. Print. doi:10.5455/egyjebb.20230921012834


APA (American Psychological Association) Style

Al-Tuwaijri, M. M. & El-Souad, . S. M. S. A. (2023) Antibacterial activity of Chaga mushroom (Inonotus obliquus) extract against Staphylococcus aureus and Salmonella typhimurium: An In Vitro Study. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 19 (2), 167-177. doi:10.5455/egyjebb.20230921012834


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