E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


Molecular characterization of enterotoxin and antibiotic resistance genes-producing Staphylococcus aureus derived from imported meat: Artemisia herb‐alba extract as an antibacterial agent

Shimaa El-Sapagh, Mohamed O. Abdel-Monem, Esraa Badr, Mohamed H. Yassine, Nesma Elsayed, Awad Y. Shala.

Abstract
Staphylococcus aureus is a major foodborne pathogen in raw and ready-to-eat meat products. In this study, the effectiveness of methanolic extracts of Artemisia herbal-alba in inhibiting pan-resistant and enterotoxic Staphylococcus aureus was investigated. The first analysis showed a high prevalence (33%) of coagulase-positive staphylococci in imported meat samples from retail stores. Examination of antibiotic resistance patterns revealed that staphylococcal antibiogram resistance profiles were diverse: five strains demonstrated resistance to at least one antimicrobial agent, fourteen isolates demonstrated multidrug resistance (MDR) and one resistant to all test antibiotics (PAN). Multiplex PCR of pan-Staphylococcus strains revealed positive serotypes for the enterotoxin genes seb, seg and sei at 665.05 bp, 277.99 bp and 460.74 bp, respectively, and a positive serotype for the mec-A gene at 538.45 bp. Compared to the tetracycline effect, methanolic extracts of Artemisia herba-alba showed significant inhibitory effects against pan-resistant Staphylococcus aureus. Real-time quantitative PCR data analysis showed that Artemisia herba-alba methanolic extracts caused a significant decrease in the expression levels of the genes mecA, mecC, sei, seg and seb. Therefore, the methanolic extract of Artemisia herba-alba has the potential to be a promising natural product to control foodborne pathogens and could be valuable for food safety applications.

Key words: Staphylococcal food poisoning, antimicrobial activity, Artemisia herba-alba, staphylococcal enterotoxins, MDR, Extracts


 
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How to Cite this Article
Pubmed Style

El-Sapagh S, Abdel-Monem MO, Badr E, Yassine MH, Elsayed N, Shala AY, . Molecular characterization of enterotoxin and antibiotic resistance genes-producing Staphylococcus aureus derived from imported meat: Artemisia herb‐alba extract as an antibacterial agent. Egypt. J. Exp. Biol. (Bot.). 2023; 19(2): 141-153. doi:10.5455/egyjebb.20230607015627


Web Style

El-Sapagh S, Abdel-Monem MO, Badr E, Yassine MH, Elsayed N, Shala AY, . Molecular characterization of enterotoxin and antibiotic resistance genes-producing Staphylococcus aureus derived from imported meat: Artemisia herb‐alba extract as an antibacterial agent. https://www.egyseb.net//?mno=156546 [Access: December 04, 2023]. doi:10.5455/egyjebb.20230607015627


AMA (American Medical Association) Style

El-Sapagh S, Abdel-Monem MO, Badr E, Yassine MH, Elsayed N, Shala AY, . Molecular characterization of enterotoxin and antibiotic resistance genes-producing Staphylococcus aureus derived from imported meat: Artemisia herb‐alba extract as an antibacterial agent. Egypt. J. Exp. Biol. (Bot.). 2023; 19(2): 141-153. doi:10.5455/egyjebb.20230607015627



Vancouver/ICMJE Style

El-Sapagh S, Abdel-Monem MO, Badr E, Yassine MH, Elsayed N, Shala AY, . Molecular characterization of enterotoxin and antibiotic resistance genes-producing Staphylococcus aureus derived from imported meat: Artemisia herb‐alba extract as an antibacterial agent. Egypt. J. Exp. Biol. (Bot.). (2023), [cited December 04, 2023]; 19(2): 141-153. doi:10.5455/egyjebb.20230607015627



Harvard Style

El-Sapagh, S., Abdel-Monem, M. O., Badr, E., Yassine, M. H., Elsayed, N., Shala, A. Y. & (2023) Molecular characterization of enterotoxin and antibiotic resistance genes-producing Staphylococcus aureus derived from imported meat: Artemisia herb‐alba extract as an antibacterial agent. Egypt. J. Exp. Biol. (Bot.), 19 (2), 141-153. doi:10.5455/egyjebb.20230607015627



Turabian Style

El-Sapagh, Shimaa, Mohamed O. Abdel-Monem, Esraa Badr, Mohamed H. Yassine, Nesma Elsayed, Awad Y. Shala, and . 2023. Molecular characterization of enterotoxin and antibiotic resistance genes-producing Staphylococcus aureus derived from imported meat: Artemisia herb‐alba extract as an antibacterial agent. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 19 (2), 141-153. doi:10.5455/egyjebb.20230607015627



Chicago Style

El-Sapagh, Shimaa, Mohamed O. Abdel-Monem, Esraa Badr, Mohamed H. Yassine, Nesma Elsayed, Awad Y. Shala, and . "Molecular characterization of enterotoxin and antibiotic resistance genes-producing Staphylococcus aureus derived from imported meat: Artemisia herb‐alba extract as an antibacterial agent." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 19 (2023), 141-153. doi:10.5455/egyjebb.20230607015627



MLA (The Modern Language Association) Style

El-Sapagh, Shimaa, Mohamed O. Abdel-Monem, Esraa Badr, Mohamed H. Yassine, Nesma Elsayed, Awad Y. Shala, and . "Molecular characterization of enterotoxin and antibiotic resistance genes-producing Staphylococcus aureus derived from imported meat: Artemisia herb‐alba extract as an antibacterial agent." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 19.2 (2023), 141-153. Print. doi:10.5455/egyjebb.20230607015627



APA (American Psychological Association) Style

El-Sapagh, S., Abdel-Monem, M. O., Badr, E., Yassine, M. H., Elsayed, N., Shala, A. Y. & (2023) Molecular characterization of enterotoxin and antibiotic resistance genes-producing Staphylococcus aureus derived from imported meat: Artemisia herb‐alba extract as an antibacterial agent. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 19 (2), 141-153. doi:10.5455/egyjebb.20230607015627