E-ISSN 2090-0503 | ISSN 1687-7497
 
2023, Vol: 19, Issue: 2
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Original Research 


SELECTIVE ISOLATION OF RARE ACTINOMYCETES FROM DIFFERENT TYPES OF EGYPTIAN SOIL

Nagwa A. Abd-Allah Sahar M. Tolba Dina M. Hatem.

Abstract
A total of 1191 actinomycetes were selectively isolated from ten soil samples collected from five different Egyptian Governorates including Alexandria, Qalubiya, Giza, Asuit and Sinai. Selective isolation was carried out by using five different pretreatment techniques including physical and chemical treatment. One hundred and eighty three isolates of rare actinomycetes were recovered. Dry heat treatment at 120ºC for 1 hour was the most effective method. Starch casein agar was the best medium which recovered the highest colony number of rare actinomycetes. The isolates were identified according to phenotypic and chemotaxonomic criteria. Genus Micromonospora was the most common and dominant non-streptomycete actinomycetes in soil. Genera such as Actinoplanes, Actinomadura, Saccharomonospora and Nocardioides were also recovered. Genus Actinoplanes was concomitant with clay soil as it was not isolated from sandy soil. Variation in the distribution of non-streptomycete indicates that soil properties play an important role for survival and propagation of non-streptomycete actinomycetes.

Key words: Rare actinomycetes, selective isolation, pretreatment, soil


 
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Pubmed Style

Nagwa A. Abd-Allah Sahar M. Tolba Dina M. Hatem. SELECTIVE ISOLATION OF RARE ACTINOMYCETES FROM DIFFERENT TYPES OF EGYPTIAN SOIL. Egypt. J. Exp. Biol. (Bot.). 2012; 8(2): 175-182.

Web Style

Nagwa A. Abd-Allah Sahar M. Tolba Dina M. Hatem. SELECTIVE ISOLATION OF RARE ACTINOMYCETES FROM DIFFERENT TYPES OF EGYPTIAN SOIL. https://www.egyseb.net//?mno=186718 [Access: August 15, 2023].

AMA (American Medical Association) Style

Nagwa A. Abd-Allah Sahar M. Tolba Dina M. Hatem. SELECTIVE ISOLATION OF RARE ACTINOMYCETES FROM DIFFERENT TYPES OF EGYPTIAN SOIL. Egypt. J. Exp. Biol. (Bot.). 2012; 8(2): 175-182.


Vancouver/ICMJE Style

Nagwa A. Abd-Allah Sahar M. Tolba Dina M. Hatem. SELECTIVE ISOLATION OF RARE ACTINOMYCETES FROM DIFFERENT TYPES OF EGYPTIAN SOIL. Egypt. J. Exp. Biol. (Bot.). (2012), [cited August 15, 2023]; 8(2): 175-182.


Harvard Style

Nagwa A. Abd-Allah Sahar M. Tolba Dina M. Hatem (2012) SELECTIVE ISOLATION OF RARE ACTINOMYCETES FROM DIFFERENT TYPES OF EGYPTIAN SOIL. Egypt. J. Exp. Biol. (Bot.), 8 (2), 175-182.


Turabian Style

Nagwa A. Abd-Allah Sahar M. Tolba Dina M. Hatem. 2012. SELECTIVE ISOLATION OF RARE ACTINOMYCETES FROM DIFFERENT TYPES OF EGYPTIAN SOIL. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 8 (2), 175-182.


Chicago Style

Nagwa A. Abd-Allah Sahar M. Tolba Dina M. Hatem. "SELECTIVE ISOLATION OF RARE ACTINOMYCETES FROM DIFFERENT TYPES OF EGYPTIAN SOIL." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 8 (2012), 175-182.


MLA (The Modern Language Association) Style

Nagwa A. Abd-Allah Sahar M. Tolba Dina M. Hatem. "SELECTIVE ISOLATION OF RARE ACTINOMYCETES FROM DIFFERENT TYPES OF EGYPTIAN SOIL." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 8.2 (2012), 175-182. Print.


APA (American Psychological Association) Style

Nagwa A. Abd-Allah Sahar M. Tolba Dina M. Hatem (2012) SELECTIVE ISOLATION OF RARE ACTINOMYCETES FROM DIFFERENT TYPES OF EGYPTIAN SOIL. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 8 (2), 175-182.


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