E-ISSN 2090-0503 | ISSN 1687-7497
 
2023, Vol: 19, Issue: 2
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Original Research 


CELLULASES APPLICATIONS IN BIOLOGICAL DE-INKING OF OLD NEWSPAPER WASTES AS CARBON SOURCE PRODUCED BY BACILLUS SUBTILIS

Essam A. Makky Tarek M. Abdel-Ghany.

Abstract
Old newspaper (ONP) wastes were used as the sole carbon source for growing Bacillus subtilis. Avicelase and carboxymethylcellulase (CMCase) cellulolytic enzymes were estimated in culture filtrate. A maximum yield of 193.4 and 121.4 % was recorded for Avicelase and CMCase respectively, comparing with 100 % yield in case of control. Avicelase optimum enzyme activity (2.822 U/ml) was recorded at 50°C, however, CMCase optimum activity (3.971 U/ml) was at 30°C. In a similar way, the optimal enzymes activities pH profile was identical and showed the maximal activity up to (2.500 and 4.364 U/ml) at pH value of 6.0 for Avicelase and CMCase, respectively. Bacillus subtilis CMCase has more activity at optimal temperature and pH than Avicelase. The enzymatic de-inking was indicated by the brightness of the sheets due to the remove of the ink particles effectively and prevents redeposition onto the fiber surfaces.

Key words: Biological de-inking, Bacillus subtilis, Avicelase, Carboxymethylcellulase.


 
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Pubmed Style

Essam A. Makky Tarek M. Abdel-Ghany. CELLULASES APPLICATIONS IN BIOLOGICAL DE-INKING OF OLD NEWSPAPER WASTES AS CARBON SOURCE PRODUCED BY BACILLUS SUBTILIS . Egypt. J. Exp. Biol. (Bot.). 2009; 5(0): 85-89.

Web Style

Essam A. Makky Tarek M. Abdel-Ghany. CELLULASES APPLICATIONS IN BIOLOGICAL DE-INKING OF OLD NEWSPAPER WASTES AS CARBON SOURCE PRODUCED BY BACILLUS SUBTILIS . https://www.egyseb.net//?mno=187201 [Access: December 05, 2023].

AMA (American Medical Association) Style

Essam A. Makky Tarek M. Abdel-Ghany. CELLULASES APPLICATIONS IN BIOLOGICAL DE-INKING OF OLD NEWSPAPER WASTES AS CARBON SOURCE PRODUCED BY BACILLUS SUBTILIS . Egypt. J. Exp. Biol. (Bot.). 2009; 5(0): 85-89.


Vancouver/ICMJE Style

Essam A. Makky Tarek M. Abdel-Ghany. CELLULASES APPLICATIONS IN BIOLOGICAL DE-INKING OF OLD NEWSPAPER WASTES AS CARBON SOURCE PRODUCED BY BACILLUS SUBTILIS . Egypt. J. Exp. Biol. (Bot.). (2009), [cited December 05, 2023]; 5(0): 85-89.


Harvard Style

Essam A. Makky Tarek M. Abdel-Ghany (2009) CELLULASES APPLICATIONS IN BIOLOGICAL DE-INKING OF OLD NEWSPAPER WASTES AS CARBON SOURCE PRODUCED BY BACILLUS SUBTILIS . Egypt. J. Exp. Biol. (Bot.), 5 (0), 85-89.


Turabian Style

Essam A. Makky Tarek M. Abdel-Ghany. 2009. CELLULASES APPLICATIONS IN BIOLOGICAL DE-INKING OF OLD NEWSPAPER WASTES AS CARBON SOURCE PRODUCED BY BACILLUS SUBTILIS . THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 5 (0), 85-89.


Chicago Style

Essam A. Makky Tarek M. Abdel-Ghany. "CELLULASES APPLICATIONS IN BIOLOGICAL DE-INKING OF OLD NEWSPAPER WASTES AS CARBON SOURCE PRODUCED BY BACILLUS SUBTILIS ." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 5 (2009), 85-89.


MLA (The Modern Language Association) Style

Essam A. Makky Tarek M. Abdel-Ghany. "CELLULASES APPLICATIONS IN BIOLOGICAL DE-INKING OF OLD NEWSPAPER WASTES AS CARBON SOURCE PRODUCED BY BACILLUS SUBTILIS ." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 5.0 (2009), 85-89. Print.


APA (American Psychological Association) Style

Essam A. Makky Tarek M. Abdel-Ghany (2009) CELLULASES APPLICATIONS IN BIOLOGICAL DE-INKING OF OLD NEWSPAPER WASTES AS CARBON SOURCE PRODUCED BY BACILLUS SUBTILIS . THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 5 (0), 85-89.


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