E-ISSN 2090-0503 | ISSN 1687-7497
 
2023, Vol: 19, Issue: 2
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Original Research 


ENHANCEMENT OF XYLANASE PRODUCTION FROM RICE STRAW BY TRICHODERMA REESEI THROUGH UV- MUTAGENESIS AND PROTOPLAST FUSION

Magda A.M. Soroor Abd El-hady M. Ghazy Abd El-Naser A. Khattab Ahmed M. Abd El-Aziz Einas H. El- Shatoury Al Zahraa A. Karam El-Din.

Abstract
An attempt to construct superior Trichoderma reesei F418 isolate for enhancing xylanase production from alkali pretreated rice straw (RS) under solid state fermentation (SSF) was conducted. Mutagenesis for T. reesei F418 in conjugation with intra-specific protoplast fusion between the selected mutants was applied to obtain hyperproducing strains. After mutagenesis by UV-irradiation, 70 mutants were recovered. Twenty eight mutants produced higher xylanase activity than the wild type (WT) with fold increase ranged from 1.1 to 2.94. Mutant Co-6 is the highest enzyme producer. According to heavy metals response, intraspecific protoplast fusion between the selected hyperproducing mutant isolates was carried out using polyethylene glycol (PEG)-6000. The fusant C6b/1 was found to be stable and the promising fusant isolate for xylanase production from alkali pretreated RS (6875 IU/gDW) and untreated RS (4122 IU/gDW) with 308% and 184.6% higher productivity, respectively, than the WT strain. The application of mutagenesis in conjunction with protoplast fusion is good and effective tools for improvement of T. reesei F418 for enhancing xylanase productivity from untreated and alkali-pretreated RS under SSF conditions.

Key words: Trichoderma, Xylanase, Rice straw, UV-mutants, Protoplast fusion, Stability


 
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How to Cite this Article
Pubmed Style

Magda A.M. Soroor Abd El-hady M. Ghazy Abd El-Naser A. Khattab Ahmed M. Abd El-Aziz Einas H. El- Shatoury Al Zahraa A. Karam El-Din. ENHANCEMENT OF XYLANASE PRODUCTION FROM RICE STRAW BY TRICHODERMA REESEI THROUGH UV- MUTAGENESIS AND PROTOPLAST FUSION . Egypt. J. Exp. Biol. (Bot.). 2014; 10(2): 125-134.

Web Style

Magda A.M. Soroor Abd El-hady M. Ghazy Abd El-Naser A. Khattab Ahmed M. Abd El-Aziz Einas H. El- Shatoury Al Zahraa A. Karam El-Din. ENHANCEMENT OF XYLANASE PRODUCTION FROM RICE STRAW BY TRICHODERMA REESEI THROUGH UV- MUTAGENESIS AND PROTOPLAST FUSION . https://www.egyseb.net//?mno=186622 [Access: December 04, 2023].

AMA (American Medical Association) Style

Magda A.M. Soroor Abd El-hady M. Ghazy Abd El-Naser A. Khattab Ahmed M. Abd El-Aziz Einas H. El- Shatoury Al Zahraa A. Karam El-Din. ENHANCEMENT OF XYLANASE PRODUCTION FROM RICE STRAW BY TRICHODERMA REESEI THROUGH UV- MUTAGENESIS AND PROTOPLAST FUSION . Egypt. J. Exp. Biol. (Bot.). 2014; 10(2): 125-134.


Vancouver/ICMJE Style

Magda A.M. Soroor Abd El-hady M. Ghazy Abd El-Naser A. Khattab Ahmed M. Abd El-Aziz Einas H. El- Shatoury Al Zahraa A. Karam El-Din. ENHANCEMENT OF XYLANASE PRODUCTION FROM RICE STRAW BY TRICHODERMA REESEI THROUGH UV- MUTAGENESIS AND PROTOPLAST FUSION . Egypt. J. Exp. Biol. (Bot.). (2014), [cited December 04, 2023]; 10(2): 125-134.


Harvard Style

Magda A.M. Soroor Abd El-hady M. Ghazy Abd El-Naser A. Khattab Ahmed M. Abd El-Aziz Einas H. El- Shatoury Al Zahraa A. Karam El-Din (2014) ENHANCEMENT OF XYLANASE PRODUCTION FROM RICE STRAW BY TRICHODERMA REESEI THROUGH UV- MUTAGENESIS AND PROTOPLAST FUSION . Egypt. J. Exp. Biol. (Bot.), 10 (2), 125-134.


Turabian Style

Magda A.M. Soroor Abd El-hady M. Ghazy Abd El-Naser A. Khattab Ahmed M. Abd El-Aziz Einas H. El- Shatoury Al Zahraa A. Karam El-Din. 2014. ENHANCEMENT OF XYLANASE PRODUCTION FROM RICE STRAW BY TRICHODERMA REESEI THROUGH UV- MUTAGENESIS AND PROTOPLAST FUSION . THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 10 (2), 125-134.


Chicago Style

Magda A.M. Soroor Abd El-hady M. Ghazy Abd El-Naser A. Khattab Ahmed M. Abd El-Aziz Einas H. El- Shatoury Al Zahraa A. Karam El-Din. "ENHANCEMENT OF XYLANASE PRODUCTION FROM RICE STRAW BY TRICHODERMA REESEI THROUGH UV- MUTAGENESIS AND PROTOPLAST FUSION ." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 10 (2014), 125-134.


MLA (The Modern Language Association) Style

Magda A.M. Soroor Abd El-hady M. Ghazy Abd El-Naser A. Khattab Ahmed M. Abd El-Aziz Einas H. El- Shatoury Al Zahraa A. Karam El-Din. "ENHANCEMENT OF XYLANASE PRODUCTION FROM RICE STRAW BY TRICHODERMA REESEI THROUGH UV- MUTAGENESIS AND PROTOPLAST FUSION ." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 10.2 (2014), 125-134. Print.


APA (American Psychological Association) Style

Magda A.M. Soroor Abd El-hady M. Ghazy Abd El-Naser A. Khattab Ahmed M. Abd El-Aziz Einas H. El- Shatoury Al Zahraa A. Karam El-Din (2014) ENHANCEMENT OF XYLANASE PRODUCTION FROM RICE STRAW BY TRICHODERMA REESEI THROUGH UV- MUTAGENESIS AND PROTOPLAST FUSION . THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 10 (2), 125-134.


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