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2023, Vol: 19, Issue: 2
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Original Research 


Microbial bioconversion of phytosterol into important androgen intermediates using Ochrobactrum anthropi strain ATCC 49188

Nanis Gamal El-Deen Allam, Ibrahim Shabaan Abdel Salam, Samia Abbas Shabana, Shaimaa Abdel Aziz Ahmed Abdel Kawy.

Abstract
Androstenedione (AD) and androstadienedione (ADD) are steroids intermediate valuable for the production of steroid medicaments. Microbial bioconversion of phytosterols to produce AD is a well researched area in synthesis of steroid hormones. The current study focused on bioconversion of β, sitosterol as a plant phytosterol into androstenedione (AD) and androstadienedione (ADD) optimized by Ochrobactrum anthropi strain (ATCC 49188). The study involved the effect of some physiological and biochemical factors affecting on the bioconversion process such as inoculums size, inoculum age, pH of the fermentation medium No.Ⅰ, (g/L) glucose 10 K2HPO4 0.75, KH2PO4 3, (NH4)2SO4 1, MgSO4 .7H2O 1, 8 hydroxyqunioline 0.8), temperature 30as well as substrate concentration. The results showed that the maximum AD (39.29%) output was obtained by using fermentation medium No.Ⅰ at pH 8; inoculum size 2ml/100 ml; 24h inoculum age, temperature 30and 15mg/100ml substrate concentration.

Key words: androstenedione (AD), androstadienedione (ADD), Phytosterols, Microbial bioconversion, Ochrobactrum anthropic


 
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How to Cite this Article
Pubmed Style

Allam NGE, Salam ISA, Shabana SA, . Microbial bioconversion of phytosterol into important androgen intermediates using Ochrobactrum anthropi strain ATCC 49188. Egypt. J. Exp. Biol. (Bot.). 2020; 16(2): 243-251. doi:10.5455/egyjebb.20201202100939

Web Style

Allam NGE, Salam ISA, Shabana SA, . Microbial bioconversion of phytosterol into important androgen intermediates using Ochrobactrum anthropi strain ATCC 49188. https://www.egyseb.net//?mno=18341 [Access: December 05, 2023]. doi:10.5455/egyjebb.20201202100939

AMA (American Medical Association) Style

Allam NGE, Salam ISA, Shabana SA, . Microbial bioconversion of phytosterol into important androgen intermediates using Ochrobactrum anthropi strain ATCC 49188. Egypt. J. Exp. Biol. (Bot.). 2020; 16(2): 243-251. doi:10.5455/egyjebb.20201202100939


Vancouver/ICMJE Style

Allam NGE, Salam ISA, Shabana SA, . Microbial bioconversion of phytosterol into important androgen intermediates using Ochrobactrum anthropi strain ATCC 49188. Egypt. J. Exp. Biol. (Bot.). (2020), [cited December 05, 2023]; 16(2): 243-251. doi:10.5455/egyjebb.20201202100939


Harvard Style

Allam, N. G. E., Salam, . I. S. A., Shabana, . S. A. & (2020) Microbial bioconversion of phytosterol into important androgen intermediates using Ochrobactrum anthropi strain ATCC 49188. Egypt. J. Exp. Biol. (Bot.), 16 (2), 243-251. doi:10.5455/egyjebb.20201202100939


Turabian Style

Allam, Nanis Gamal El-Deen, Ibrahim Shabaan Abdel Salam, Samia Abbas Shabana, and Shaimaa Abdel Aziz Ahmed Abdel Kawy. 2020. Microbial bioconversion of phytosterol into important androgen intermediates using Ochrobactrum anthropi strain ATCC 49188. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 16 (2), 243-251. doi:10.5455/egyjebb.20201202100939


Chicago Style

Allam, Nanis Gamal El-Deen, Ibrahim Shabaan Abdel Salam, Samia Abbas Shabana, and Shaimaa Abdel Aziz Ahmed Abdel Kawy. "Microbial bioconversion of phytosterol into important androgen intermediates using Ochrobactrum anthropi strain ATCC 49188." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 16 (2020), 243-251. doi:10.5455/egyjebb.20201202100939


MLA (The Modern Language Association) Style

Allam, Nanis Gamal El-Deen, Ibrahim Shabaan Abdel Salam, Samia Abbas Shabana, and Shaimaa Abdel Aziz Ahmed Abdel Kawy. "Microbial bioconversion of phytosterol into important androgen intermediates using Ochrobactrum anthropi strain ATCC 49188." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 16.2 (2020), 243-251. Print. doi:10.5455/egyjebb.20201202100939


APA (American Psychological Association) Style

Allam, N. G. E., Salam, . I. S. A., Shabana, . S. A. & (2020) Microbial bioconversion of phytosterol into important androgen intermediates using Ochrobactrum anthropi strain ATCC 49188. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 16 (2), 243-251. doi:10.5455/egyjebb.20201202100939


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