E-ISSN 2090-0503 | ISSN 2090-0511
 
2023|19|1, Vol: , Issue:
/0Current Issue Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Research 


Histological and immunohistochemical studies on the effect of zinc oxide nanoparticles on the cerebellum of newborn and adult rats and the possible protective role of EDTA

Siham B. Salem, Sabry A. El-Naggar, Ramadan M. Kandyel, Marwa M. El-Sharkawy.

Abstract
Zinc oxide nanoparticles (ZnO NPs) are involved in different applications such as nutrition, while, it causes damage to different organs of the body including the brain. This study aimed to compare between the effect of ZnO NPs on newborn and adult rats´ cerebellum and to evaluate the protective role of Ethylenediaminetetraacetic acid (EDTA) against ZnO NPs toxicity. Thirty-two newborn (NBM)and thirty-two adult male (ADM) Sprague Dawley rats are used. Four groups (n = 8) of each are divided as following; Gp1: is served as control, Gp2: injected intraperitoneal (IP) with ZnO NPs (3 g/ kg). Gp3: injected IP with EDTA (500 mg/ kg) for 7 days. Gp4: injected with ZnO NPs and treated with EDTA as in Gp3. Brain tissue is prepared for biochemical and histopathological investigations. Newborn and adult rats injected with ZnO NPs showed degeneration of purkinje and granular cells in cerebellum tissue confirmed with high expression of caspase-3, while, treatment with EDTA post ZnO NPs showed lightly improvement. The body weights and relative brain weights is decrease in all the rats treated with ZnO NPs. In addition, our current study proved that the effect of ZnO NPs on ADM rats is more than on the NBM ones. Collectively, ZnO NPs injection caused damage of the cerebellum in newborn and adult rats, however, the treatment with EDTA stopped somewhat this damage.

Key words: Histological and immunohistochemical features, newborn, adult rats, cerebellum, zinc oxide nanoparticles, EDTA


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Siham B. Salem
Articles by Sabry A. El-Naggar
Articles by Ramadan M. Kandyel
Articles by Marwa M. El-Sharkawy
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Salem SB, SAE, RMK, El-Sharkawy MM, . Histological and immunohistochemical studies on the effect of zinc oxide nanoparticles on the cerebellum of newborn and adult rats and the possible protective role of EDTA. Egypt. J. Exp. Biol. (Zoo.). 2023; 19(1): 15-27. doi:10.5455/egysebz.20221222014955

Web Style

Salem SB, SAE, RMK, El-Sharkawy MM, . Histological and immunohistochemical studies on the effect of zinc oxide nanoparticles on the cerebellum of newborn and adult rats and the possible protective role of EDTA. https://www.egyseb.net/ejebz/?mno=133209 [Access: March 29, 2023]. doi:10.5455/egysebz.20221222014955

AMA (American Medical Association) Style

Salem SB, SAE, RMK, El-Sharkawy MM, . Histological and immunohistochemical studies on the effect of zinc oxide nanoparticles on the cerebellum of newborn and adult rats and the possible protective role of EDTA. Egypt. J. Exp. Biol. (Zoo.). 2023; 19(1): 15-27. doi:10.5455/egysebz.20221222014955


Vancouver/ICMJE Style

Salem SB, SAE, RMK, El-Sharkawy MM, . Histological and immunohistochemical studies on the effect of zinc oxide nanoparticles on the cerebellum of newborn and adult rats and the possible protective role of EDTA. Egypt. J. Exp. Biol. (Zoo.). (2023), [cited March 29, 2023]; 19(1): 15-27. doi:10.5455/egysebz.20221222014955


Harvard Style

Salem, S. B., , S. A. E., , R. M. K., El-Sharkawy, M. M. & (2023) Histological and immunohistochemical studies on the effect of zinc oxide nanoparticles on the cerebellum of newborn and adult rats and the possible protective role of EDTA. Egypt. J. Exp. Biol. (Zoo.), 19 (1), 15-27. doi:10.5455/egysebz.20221222014955


Turabian Style

Salem, Siham B., Sabry A. El-Naggar, Ramadan M. Kandyel, Marwa M. El-Sharkawy, and . 2023. Histological and immunohistochemical studies on the effect of zinc oxide nanoparticles on the cerebellum of newborn and adult rats and the possible protective role of EDTA. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology), 19 (1), 15-27. doi:10.5455/egysebz.20221222014955


Chicago Style

Salem, Siham B., Sabry A. El-Naggar, Ramadan M. Kandyel, Marwa M. El-Sharkawy, and . "Histological and immunohistochemical studies on the effect of zinc oxide nanoparticles on the cerebellum of newborn and adult rats and the possible protective role of EDTA." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology) 19 (2023), 15-27. doi:10.5455/egysebz.20221222014955


MLA (The Modern Language Association) Style

Salem, Siham B., Sabry A. El-Naggar, Ramadan M. Kandyel, Marwa M. El-Sharkawy, and . "Histological and immunohistochemical studies on the effect of zinc oxide nanoparticles on the cerebellum of newborn and adult rats and the possible protective role of EDTA." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology) 19.1 (2023), 15-27. Print. doi:10.5455/egysebz.20221222014955


APA (American Psychological Association) Style

Salem, S. B., , S. A. E., , R. M. K., El-Sharkawy, M. M. & (2023) Histological and immunohistochemical studies on the effect of zinc oxide nanoparticles on the cerebellum of newborn and adult rats and the possible protective role of EDTA. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology), 19 (1), 15-27. doi:10.5455/egysebz.20221222014955


Author Login Reviewer Login About Publisher Editorial Policies Peer Review Policy Editorial & Peer Review Process Author's Rights and Obligations Publication Ethics and Publication Malpractice Statement Conflict of Interest Policy Plagiarism Policy Protection of Research Participants (Statement On Human And Animal Rights) Privacy Policy Publishing Ethics Corrections, Retractions & Expressions of Concern Self-Archiving Policies Statement of Informed Consent Advertising Policy Terms of Use License Information
This is an open access journal which means that all content is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles in this journal without asking prior permission from the publisher or the author. This is in accordance with the Budapest Open Access Initiative (BOAI) definition of open access.
The articles in THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology) are open access articles licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc-sa/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
Copyright � 2023 THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology) All Rights Reserved. Subject to change without notice from or liability to THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology).
For best results, please use Internet Explorer or Google Chrome

Contact Information