E-ISSN 2090-0503 | ISSN 1687-7497
 
2022, Vol: 18, Issue: 1
18/1Current Issue Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Research 


ISOLATION, IDENTIFICATION, AND DETECTION OF LIGNINOLYTIC CAPABILITY OF SOME FUNGI ISOLATED FROM SOIL SAMPLES IN EGYPT.

Mohamed Yasser bedaiwy, Omyma Ahmed Awadalla, Metwally Abd-El-Azeem Metwally, Amira Marzouk Abd Al-Samad.

Abstract
In the present study, twenty-seven fungal species were isolated, purified, and identified from five mixed soil samples collected from Tanta City, Gharbia Governorate, Egypt. The isolated fungal species belonging to nine genera i.e., Aspergillus, Cephalosporium, Fusarium, Penicillium, Botritrichom, Trichoderma, Alternaria, Cladosporium and Scopulariopsis, in addition to two white rot fungi Ganoderma lucidum and Lentinula edodes obtained from Mushroom Laboratory Culture Collection (MLCC), El-Giza, Egypt. The ligninolytic activities of the isolated fungi were evaluated on PDA plates containing 0.02% guaiacol as a chromogenic reagent. Out of the twenty-nine fungal species tested, only three fungi G. lucidum, L. edodes and T. harzianum (isolate 2) showed ligninolytic potential and reddish-brown color zone formed due to guaiacol oxidation. The three positive fungal strains were screened quantitively for Laccase (Lac; E.C. 1.10.3.2), Manganese dependent peroxidase (MnP; E.C. 1.11.1.13), and Lignin peroxidase (LiP; E.C. 1.11.1.14) activities. The highest total ligninase activity was recorded by G. lucidum followed by L. edodes and T. harzianum, respectively.

Key words: Ligninolytic activity, Screening, Guaiacol, ligninases, ligninolytic enzymes, Laccase


 
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How to Cite this Article
Pubmed Style

bedaiwy MY, Awadalla OA, Metwally MA, Al-Samad AMA. ISOLATION, IDENTIFICATION, AND DETECTION OF LIGNINOLYTIC CAPABILITY OF SOME FUNGI ISOLATED FROM SOIL SAMPLES IN EGYPT.. Egypt. J. Exp. Biol. (Bot.). 2022; 18(2): 197-204. doi:10.5455/egyjebb.20220629084930

Web Style

bedaiwy MY, Awadalla OA, Metwally MA, Al-Samad AMA. ISOLATION, IDENTIFICATION, AND DETECTION OF LIGNINOLYTIC CAPABILITY OF SOME FUNGI ISOLATED FROM SOIL SAMPLES IN EGYPT.. https://www.egyseb.net//?mno=72262 [Access: August 13, 2022]. doi:10.5455/egyjebb.20220629084930

AMA (American Medical Association) Style

bedaiwy MY, Awadalla OA, Metwally MA, Al-Samad AMA. ISOLATION, IDENTIFICATION, AND DETECTION OF LIGNINOLYTIC CAPABILITY OF SOME FUNGI ISOLATED FROM SOIL SAMPLES IN EGYPT.. Egypt. J. Exp. Biol. (Bot.). 2022; 18(2): 197-204. doi:10.5455/egyjebb.20220629084930


Vancouver/ICMJE Style

bedaiwy MY, Awadalla OA, Metwally MA, Al-Samad AMA. ISOLATION, IDENTIFICATION, AND DETECTION OF LIGNINOLYTIC CAPABILITY OF SOME FUNGI ISOLATED FROM SOIL SAMPLES IN EGYPT.. Egypt. J. Exp. Biol. (Bot.). (2022), [cited August 13, 2022]; 18(2): 197-204. doi:10.5455/egyjebb.20220629084930


Harvard Style

bedaiwy, M. Y., Awadalla, . O. A., Metwally, . M. A. & Al-Samad, . A. M. A. (2022) ISOLATION, IDENTIFICATION, AND DETECTION OF LIGNINOLYTIC CAPABILITY OF SOME FUNGI ISOLATED FROM SOIL SAMPLES IN EGYPT.. Egypt. J. Exp. Biol. (Bot.), 18 (2), 197-204. doi:10.5455/egyjebb.20220629084930


Turabian Style

bedaiwy, Mohamed Yasser, Omyma Ahmed Awadalla, Metwally Abd-El-Azeem Metwally, and Amira Marzouk Abd Al-Samad. 2022. ISOLATION, IDENTIFICATION, AND DETECTION OF LIGNINOLYTIC CAPABILITY OF SOME FUNGI ISOLATED FROM SOIL SAMPLES IN EGYPT.. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 18 (2), 197-204. doi:10.5455/egyjebb.20220629084930


Chicago Style

bedaiwy, Mohamed Yasser, Omyma Ahmed Awadalla, Metwally Abd-El-Azeem Metwally, and Amira Marzouk Abd Al-Samad. "ISOLATION, IDENTIFICATION, AND DETECTION OF LIGNINOLYTIC CAPABILITY OF SOME FUNGI ISOLATED FROM SOIL SAMPLES IN EGYPT.." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 18 (2022), 197-204. doi:10.5455/egyjebb.20220629084930


MLA (The Modern Language Association) Style

bedaiwy, Mohamed Yasser, Omyma Ahmed Awadalla, Metwally Abd-El-Azeem Metwally, and Amira Marzouk Abd Al-Samad. "ISOLATION, IDENTIFICATION, AND DETECTION OF LIGNINOLYTIC CAPABILITY OF SOME FUNGI ISOLATED FROM SOIL SAMPLES IN EGYPT.." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 18.2 (2022), 197-204. Print. doi:10.5455/egyjebb.20220629084930


APA (American Psychological Association) Style

bedaiwy, M. Y., Awadalla, . O. A., Metwally, . M. A. & Al-Samad, . A. M. A. (2022) ISOLATION, IDENTIFICATION, AND DETECTION OF LIGNINOLYTIC CAPABILITY OF SOME FUNGI ISOLATED FROM SOIL SAMPLES IN EGYPT.. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 18 (2), 197-204. doi:10.5455/egyjebb.20220629084930


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