E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


The effect of different growth conditions on the biomass and chemical constituents of Chlorella vulgaris

Mostafa El-Sheekh, Sabha El-Sabagh, Ghada Abou Elsoud, Amany Elbeltagy.

Abstract
Chlorella vulgaris is a photosynthetic unicellular green eukaryotic alga. It is widely used as food additive and in the pharmaceutical and cosmetics industry. It contains proteins, carotenoids, lipids, immunostimulator compounds, polysaccharides, vitamins, antioxidants, and minerals. The present study was conducted to investigate five different synthetic media such as BG-11, Chu- 10, Boldís Basal medium, Allen, and Kuhlís medium on the optical density, cell count, chlorophyll-a content, protein content and carbohydrate content of Chlorella vulgaris. Kuhlís medium had the best growth followed by Allen, BG-11, Boldís Basal medium, and the Chu- 10 medium. The highest optical density, cell count, and chlorophyll- a content were obtained at pH 12 as compared with a control. NH4Cl was the best nitrogen source for Chlorella vulgaris which was cultivated in Kuhlís medium as compared with KNO3 as a control. Aeration of algal culture and using sunlight as a light source increased growth and chlorophyll-a content over nonaerated culture using artificial light.

Key words: Chlorella vulgaris, growth conditions, chlorophyll- a content, nitrogen sources, aeration.


 
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How to Cite this Article
Pubmed Style

Mostafa El-Sheekh, Sabha El-Sabagh, Ghada Abou Elsoud, Amany Elbeltagy. The effect of different growth conditions on the biomass and chemical constituents of Chlorella vulgaris. Egypt. J. Exp. Biol. (Bot.). 2018; 14(1): 121-131. doi:10.5455/egyjebb.20180402100901


Web Style

Mostafa El-Sheekh, Sabha El-Sabagh, Ghada Abou Elsoud, Amany Elbeltagy. The effect of different growth conditions on the biomass and chemical constituents of Chlorella vulgaris. http://www.egyseb.net//?mno=295540 [Access: November 03, 2020]. doi:10.5455/egyjebb.20180402100901


AMA (American Medical Association) Style

Mostafa El-Sheekh, Sabha El-Sabagh, Ghada Abou Elsoud, Amany Elbeltagy. The effect of different growth conditions on the biomass and chemical constituents of Chlorella vulgaris. Egypt. J. Exp. Biol. (Bot.). 2018; 14(1): 121-131. doi:10.5455/egyjebb.20180402100901



Vancouver/ICMJE Style

Mostafa El-Sheekh, Sabha El-Sabagh, Ghada Abou Elsoud, Amany Elbeltagy. The effect of different growth conditions on the biomass and chemical constituents of Chlorella vulgaris. Egypt. J. Exp. Biol. (Bot.). (2018), [cited November 03, 2020]; 14(1): 121-131. doi:10.5455/egyjebb.20180402100901



Harvard Style

Mostafa El-Sheekh, Sabha El-Sabagh, Ghada Abou Elsoud, Amany Elbeltagy (2018) The effect of different growth conditions on the biomass and chemical constituents of Chlorella vulgaris. Egypt. J. Exp. Biol. (Bot.), 14 (1), 121-131. doi:10.5455/egyjebb.20180402100901



Turabian Style

Mostafa El-Sheekh, Sabha El-Sabagh, Ghada Abou Elsoud, Amany Elbeltagy. 2018. The effect of different growth conditions on the biomass and chemical constituents of Chlorella vulgaris. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 14 (1), 121-131. doi:10.5455/egyjebb.20180402100901



Chicago Style

Mostafa El-Sheekh, Sabha El-Sabagh, Ghada Abou Elsoud, Amany Elbeltagy. "The effect of different growth conditions on the biomass and chemical constituents of Chlorella vulgaris." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 14 (2018), 121-131. doi:10.5455/egyjebb.20180402100901



MLA (The Modern Language Association) Style

Mostafa El-Sheekh, Sabha El-Sabagh, Ghada Abou Elsoud, Amany Elbeltagy. "The effect of different growth conditions on the biomass and chemical constituents of Chlorella vulgaris." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 14.1 (2018), 121-131. Print. doi:10.5455/egyjebb.20180402100901



APA (American Psychological Association) Style

Mostafa El-Sheekh, Sabha El-Sabagh, Ghada Abou Elsoud, Amany Elbeltagy (2018) The effect of different growth conditions on the biomass and chemical constituents of Chlorella vulgaris. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 14 (1), 121-131. doi:10.5455/egyjebb.20180402100901