E-ISSN 2090-0503 | ISSN 1687-7497
 
2021, Vol: 17, Issue: 1
17/1Current Issue Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Research 


Physiological, cytological and molecular analysis of PVYNTN-infected potato cultivars

Mohamed Atef Nasr-Eldin, Badawi Abdelsalam Othman, Allam Arafat Megahed, Samar S El-Masry, Abeer A Faiesal.

Cited by (2)

Abstract
The tuber necrotic strain of Potato Virus Y (PVYNTN) is considered the most aggressive strain of PVY. Effects of PVYNTN on some metabolic activities and cytological characteristics of three potato cultivars Lady Rosetta, Mondial and Santana were investigated. Virus concentration, infection percentage and disease severity were also estimated. Potato cultivars differed in their susceptibilities to PVYNTN infection, whereas various levels of apical necrosis with different disease severity 30, 60 and 100% were recorded for Mondial, Santana and Lady Rosetta cultivars respectively. The lowest amount of leaf pigments was found in PVYNTN-infected potato cv. Santana. Total phenolic and total soluble sugars contents were highly accumulated in PVYNTN-infected leaves compared to healthy ones. A noticeable increased level of sodium cation was detected in PVYNTN-infected leaves of potato cv. Lady Rosetta. Electron microscopy analysis showed changes in nuclei, mitochondria, cell walls, and chloroplasts in PVYNTN-infected cells of potato cultivars compared with healthy. Genetic variations among potato cultivars under PVYNTN infection were detected by Inter Simple Sequence Repeat-Polymerase Chain Reaction (ISSR-PCR) analysis. The ISSR primers amplified 57 bands, and 52 showed polymorphism (91.23%). According to our results, potato cvs. Lady Rosetta and Santana were highly susceptible to PVYNTN infection while, Mondial was moderately resistant.

Key words: PVYNTN, disease severity, physiology, cytology, ISSR-PCR, potato cultivars.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Mohamed Atef Nasr-Eldin
Articles by Badawi Abdelsalam Othman
Articles by Allam Arafat Megahed
Articles by Samar S El-Masry
Articles by Abeer A Faiesal
on Google
on Google Scholar


This Article Cited By the following articles

Induction of potato systemic resistance against the potato virus Y (PVYNTN), using crude filtrates of Streptomyces spp. under greenhouse conditions
Egypt J Biol Pest Control 2019; 29(1): .

1
 
Monitoring of co-infection virus and virus-like naturally in sweet pepper plant
Archives of Phytopathology and Plant Protection 2019; 52(3-4): 333.

2
 
How to Cite this Article
Pubmed Style

Mohamed Atef Nasr-Eldin, Badawi Abdelsalam Othman, Allam Arafat Megahed, Samar S El-Masry, Abeer A Faiesal. Physiological, cytological and molecular analysis of PVYNTN-infected potato cultivars. Egypt. J. Exp. Biol. (Bot.). 2018; 14(1): 171-185. doi:10.5455/egyjebb.20180222091921

Web Style

Mohamed Atef Nasr-Eldin, Badawi Abdelsalam Othman, Allam Arafat Megahed, Samar S El-Masry, Abeer A Faiesal. Physiological, cytological and molecular analysis of PVYNTN-infected potato cultivars. http://www.egyseb.net//?mno=291820 [Access: November 03, 2020]. doi:10.5455/egyjebb.20180222091921

AMA (American Medical Association) Style

Mohamed Atef Nasr-Eldin, Badawi Abdelsalam Othman, Allam Arafat Megahed, Samar S El-Masry, Abeer A Faiesal. Physiological, cytological and molecular analysis of PVYNTN-infected potato cultivars. Egypt. J. Exp. Biol. (Bot.). 2018; 14(1): 171-185. doi:10.5455/egyjebb.20180222091921


Vancouver/ICMJE Style

Mohamed Atef Nasr-Eldin, Badawi Abdelsalam Othman, Allam Arafat Megahed, Samar S El-Masry, Abeer A Faiesal. Physiological, cytological and molecular analysis of PVYNTN-infected potato cultivars. Egypt. J. Exp. Biol. (Bot.). (2018), [cited November 03, 2020]; 14(1): 171-185. doi:10.5455/egyjebb.20180222091921


Harvard Style

Mohamed Atef Nasr-Eldin, Badawi Abdelsalam Othman, Allam Arafat Megahed, Samar S El-Masry, Abeer A Faiesal (2018) Physiological, cytological and molecular analysis of PVYNTN-infected potato cultivars. Egypt. J. Exp. Biol. (Bot.), 14 (1), 171-185. doi:10.5455/egyjebb.20180222091921


Turabian Style

Mohamed Atef Nasr-Eldin, Badawi Abdelsalam Othman, Allam Arafat Megahed, Samar S El-Masry, Abeer A Faiesal. 2018. Physiological, cytological and molecular analysis of PVYNTN-infected potato cultivars. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 14 (1), 171-185. doi:10.5455/egyjebb.20180222091921


Chicago Style

Mohamed Atef Nasr-Eldin, Badawi Abdelsalam Othman, Allam Arafat Megahed, Samar S El-Masry, Abeer A Faiesal. "Physiological, cytological and molecular analysis of PVYNTN-infected potato cultivars." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 14 (2018), 171-185. doi:10.5455/egyjebb.20180222091921


MLA (The Modern Language Association) Style

Mohamed Atef Nasr-Eldin, Badawi Abdelsalam Othman, Allam Arafat Megahed, Samar S El-Masry, Abeer A Faiesal. "Physiological, cytological and molecular analysis of PVYNTN-infected potato cultivars." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 14.1 (2018), 171-185. Print. doi:10.5455/egyjebb.20180222091921


APA (American Psychological Association) Style

Mohamed Atef Nasr-Eldin, Badawi Abdelsalam Othman, Allam Arafat Megahed, Samar S El-Masry, Abeer A Faiesal (2018) Physiological, cytological and molecular analysis of PVYNTN-infected potato cultivars. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 14 (1), 171-185. doi:10.5455/egyjebb.20180222091921


Author Login Reviewer Login About Publisher Editorial Policies Peer Review Policy Editorial & Peer Review Process Author's Rights and Obligations Publication Ethics and Publication Malpractice Statement Conflict of Interest Policy Plagiarism Policy Protection of Research Participants (Statement On Human And Animal Rights) Privacy Policy Publication Ethics and Publication Malpractice Statement Corrections, Retractions & Expressions of Concern Self-Archiving Policies Statement of Informed Consent Advertising Policy Terms of Use License Information Copyright Information
This is an open access journal which means that all content is freely available without charge to the user or his/her institution. Users are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles in this journal without asking prior permission from the publisher or the author. This is in accordance with the Budapest Open Access Initiative (BOAI) definition of open access.
The articles in THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) are open access articles licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc-sa/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
Copyright © 2021 THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) All Rights Reserved. Subject to change without notice from or liability to THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany).
For best results, please use Internet Explorer or Google Chrome

Contact Information