E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


Bioactivity Enhancement with Microbial Silver Nanoparticles ‎Produced and Characterized from Streptomyces and Trichoderma

Amr & 8206; El-Waseif, Nevin Ibrahim, Shimaa Ahmed& 8206;, Nagwa Abdallah.

Abstract
Two microbial isolates selected from Egyptian soil were used for ‎biological synthesis of silver nanoparticles using silver nitrate solution. ‎The selection of these two microorganisms was depending on the ‎ability of actinomycetes and fungi to AgNPs synthesis and production ‎of antimicrobial compounds. Results recorded that the dry weight of ‎AgNPs produced from selected Streptomyces clavuligerus and ‎Trichoderma harzianum AUMC 5408 were 66.5 and 11.0 mg/l ‎respectively. Description of AgNPs from Streptomyces clavuligerus ‎supernatant and Trichoderma harzianum AUMC 5408 mycelia were ‎carried out. Results of UV-analysis showed spectrum located between ‎‎420-440 nm for both culture filtrate and mycelium respectively ‎characteristics of AgNPs. Also, the Fourier Transform Infrared ‎Spectroscopy (FTIR) analysis was studied. The average size of AgNPs ‎were between rang from 2.0 to 4.5 nm in case of Streptomyces ‎clavuligerus, but 5.33-29.46 nm in case of Trichoderma harzianum ‎AUMC 5408. Enhancement of the antimicrobial activity of crude ‎antimicrobial compounds with Silver Nanoparticles was assayed. The ‎results revealed that addition of AgNPs to the culture filtrate of ‎Streptomyces clavuligerus and Trichoderma harzianum AUMC 5408 ‎that have antimicrobial activity increased the diameter of inhibition ‎zone between 3-5 mm in both cases.‎

Key words: Silver Nanoparticles, UV-visible, FTIR analysis, TEM and Antimicrobial ‎activity.‎


 
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How to Cite this Article
Pubmed Style

Aa, , Abdallah ,N. Bioactivity Enhancement with Microbial Silver Nanoparticles ‎Produced and Characterized from Streptomyces and Trichoderma. Egypt. J. Exp. Biol. (Bot.). 2017; 13(2): 439-445. doi:10.5455/egyjebb.20171108083304


Web Style

Aa, , Abdallah ,N. Bioactivity Enhancement with Microbial Silver Nanoparticles ‎Produced and Characterized from Streptomyces and Trichoderma. http://www.egyseb.net//?mno=276870 [Access: November 04, 2020]. doi:10.5455/egyjebb.20171108083304


AMA (American Medical Association) Style

Aa, , Abdallah ,N. Bioactivity Enhancement with Microbial Silver Nanoparticles ‎Produced and Characterized from Streptomyces and Trichoderma. Egypt. J. Exp. Biol. (Bot.). 2017; 13(2): 439-445. doi:10.5455/egyjebb.20171108083304



Vancouver/ICMJE Style

Aa, , Abdallah ,N. Bioactivity Enhancement with Microbial Silver Nanoparticles ‎Produced and Characterized from Streptomyces and Trichoderma. Egypt. J. Exp. Biol. (Bot.). (2017), [cited November 04, 2020]; 13(2): 439-445. doi:10.5455/egyjebb.20171108083304



Harvard Style

, A. a. ., & Abdallah, ,. N. (2017) Bioactivity Enhancement with Microbial Silver Nanoparticles ‎Produced and Characterized from Streptomyces and Trichoderma. Egypt. J. Exp. Biol. (Bot.), 13 (2), 439-445. doi:10.5455/egyjebb.20171108083304



Turabian Style

, Amr and , El-Waseif, Nevin Ibrahim, Shimaa Ahmed and, and , Nagwa Abdallah. 2017. Bioactivity Enhancement with Microbial Silver Nanoparticles ‎Produced and Characterized from Streptomyces and Trichoderma. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 13 (2), 439-445. doi:10.5455/egyjebb.20171108083304



Chicago Style

, Amr and , El-Waseif, Nevin Ibrahim, Shimaa Ahmed and, and , Nagwa Abdallah. "Bioactivity Enhancement with Microbial Silver Nanoparticles ‎Produced and Characterized from Streptomyces and Trichoderma." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 13 (2017), 439-445. doi:10.5455/egyjebb.20171108083304



MLA (The Modern Language Association) Style

, Amr and , El-Waseif, Nevin Ibrahim, Shimaa Ahmed and, and , Nagwa Abdallah. "Bioactivity Enhancement with Microbial Silver Nanoparticles ‎Produced and Characterized from Streptomyces and Trichoderma." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 13.2 (2017), 439-445. Print. doi:10.5455/egyjebb.20171108083304



APA (American Psychological Association) Style

, A. a. ., & Abdallah, ,. N. (2017) Bioactivity Enhancement with Microbial Silver Nanoparticles ‎Produced and Characterized from Streptomyces and Trichoderma. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 13 (2), 439-445. doi:10.5455/egyjebb.20171108083304