E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


Fermentative production of Ergosterol using Sacharomyces boulardii ATCC 236

Ibrahim Shabaan Abdelsalam, Nehad Zaki Adham, yosseria Mohamed shetiaia.

Abstract
Summary
Ergosterol is an economically important metabolite produced by yeast cells. In this study the production of ergosterol was investigated using batch and fed batch conditions. The production process was carried out in7 L bioreactor under optimized growth fermentation conditions involving growth medium containing the following constituents (g%) glucose 8, corn steep liquor 10, urea 0.1, KH2PO4 0.6, MgSO40.1, and NaNO3 0.2 ; pH 9 and inoculums size 3ml/100ml medium. The results showed significant increase in ergosterol production from 342 to488mg/l by increasing the agitation speed from 400 rpm to 600rpm. The fed batch studies showed that glucose feeding increases ergosterol productivity more than 80% under fed batch fermentation comparing to the batch fermentation. However, corn steep liquor feeding at conc. of 10g% gave the maximum amount of ergosterol (1253mg/l) during the exponential yeast growth phase.

Key words: Keywards, Production, Ergosterol, batch ,fed batch fermentation


 
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How to Cite this Article
Pubmed Style

Ibrahim Shabaan Abdelsalam, Nehad Zaki Adham, yosseria Mohamed shetiaia. Fermentative production of Ergosterol using Sacharomyces boulardii ATCC 236. Egypt. J. Exp. Biol. (Bot.). 2017; 13(2): 361-366. doi:10.5455/egyjebb.20170913073156


Web Style

Ibrahim Shabaan Abdelsalam, Nehad Zaki Adham, yosseria Mohamed shetiaia. Fermentative production of Ergosterol using Sacharomyces boulardii ATCC 236. http://www.egyseb.net//?mno=275330 [Access: November 04, 2020]. doi:10.5455/egyjebb.20170913073156


AMA (American Medical Association) Style

Ibrahim Shabaan Abdelsalam, Nehad Zaki Adham, yosseria Mohamed shetiaia. Fermentative production of Ergosterol using Sacharomyces boulardii ATCC 236. Egypt. J. Exp. Biol. (Bot.). 2017; 13(2): 361-366. doi:10.5455/egyjebb.20170913073156



Vancouver/ICMJE Style

Ibrahim Shabaan Abdelsalam, Nehad Zaki Adham, yosseria Mohamed shetiaia. Fermentative production of Ergosterol using Sacharomyces boulardii ATCC 236. Egypt. J. Exp. Biol. (Bot.). (2017), [cited November 04, 2020]; 13(2): 361-366. doi:10.5455/egyjebb.20170913073156



Harvard Style

Ibrahim Shabaan Abdelsalam, Nehad Zaki Adham, yosseria Mohamed shetiaia (2017) Fermentative production of Ergosterol using Sacharomyces boulardii ATCC 236. Egypt. J. Exp. Biol. (Bot.), 13 (2), 361-366. doi:10.5455/egyjebb.20170913073156



Turabian Style

Ibrahim Shabaan Abdelsalam, Nehad Zaki Adham, yosseria Mohamed shetiaia. 2017. Fermentative production of Ergosterol using Sacharomyces boulardii ATCC 236. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 13 (2), 361-366. doi:10.5455/egyjebb.20170913073156



Chicago Style

Ibrahim Shabaan Abdelsalam, Nehad Zaki Adham, yosseria Mohamed shetiaia. "Fermentative production of Ergosterol using Sacharomyces boulardii ATCC 236." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 13 (2017), 361-366. doi:10.5455/egyjebb.20170913073156



MLA (The Modern Language Association) Style

Ibrahim Shabaan Abdelsalam, Nehad Zaki Adham, yosseria Mohamed shetiaia. "Fermentative production of Ergosterol using Sacharomyces boulardii ATCC 236." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 13.2 (2017), 361-366. Print. doi:10.5455/egyjebb.20170913073156



APA (American Psychological Association) Style

Ibrahim Shabaan Abdelsalam, Nehad Zaki Adham, yosseria Mohamed shetiaia (2017) Fermentative production of Ergosterol using Sacharomyces boulardii ATCC 236. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 13 (2), 361-366. doi:10.5455/egyjebb.20170913073156