E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


Production of an extracellular carboxymethyl cellulase from Exiguobacterium aurantiacum AN2 by submerged fermentation

Noura Hagaggi.

Abstract
Exiguobacterium aurantiacum AN2 which was isolated from an ancient archaeological manuscript kept in Al- Azhar library at Cairo, Egypt which exhibited a potent ability to produce an extracellular carboxymethyl cellulase (CMCase). The effect of various parameters on the production of CMCase by the strain was studied. Maximum production was obtained in medium contained 0.5 % (w/v) carboxymethyl cellulose as a substrate. Lactose, carboxymethyl cellulose and yeast extract were the best carbon and nitrogen sources promoted enzyme production respectively. Maximum production was achieved at pH 7-10, temperature 37 0C, shaking rate 150 rpm, 2 % (v/v) inoculum size, after 96 hours of incubation period. Filter paper was efficiently degraded by the present enzyme than cotton.

Key words: Exiguobacterium aurantiacum, production, carboxymethyl cellulase, parameters, Maximum


 
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Pubmed Style

Noura Hagaggi. Production of an extracellular carboxymethyl cellulase from Exiguobacterium aurantiacum AN2 by submerged fermentation. Egypt. J. Exp. Biol. (Bot.). 2018; 14(1): -. doi:10.5455/egyjebb.20170807070652


Web Style

Noura Hagaggi. Production of an extracellular carboxymethyl cellulase from Exiguobacterium aurantiacum AN2 by submerged fermentation. https://www.egyseb.net//?mno=273894 [Access: December 05, 2023]. doi:10.5455/egyjebb.20170807070652


AMA (American Medical Association) Style

Noura Hagaggi. Production of an extracellular carboxymethyl cellulase from Exiguobacterium aurantiacum AN2 by submerged fermentation. Egypt. J. Exp. Biol. (Bot.). 2018; 14(1): -. doi:10.5455/egyjebb.20170807070652



Vancouver/ICMJE Style

Noura Hagaggi. Production of an extracellular carboxymethyl cellulase from Exiguobacterium aurantiacum AN2 by submerged fermentation. Egypt. J. Exp. Biol. (Bot.). (2018), [cited December 05, 2023]; 14(1): -. doi:10.5455/egyjebb.20170807070652



Harvard Style

Noura Hagaggi (2018) Production of an extracellular carboxymethyl cellulase from Exiguobacterium aurantiacum AN2 by submerged fermentation. Egypt. J. Exp. Biol. (Bot.), 14 (1), -. doi:10.5455/egyjebb.20170807070652



Turabian Style

Noura Hagaggi. 2018. Production of an extracellular carboxymethyl cellulase from Exiguobacterium aurantiacum AN2 by submerged fermentation. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 14 (1), -. doi:10.5455/egyjebb.20170807070652



Chicago Style

Noura Hagaggi. "Production of an extracellular carboxymethyl cellulase from Exiguobacterium aurantiacum AN2 by submerged fermentation." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 14 (2018), -. doi:10.5455/egyjebb.20170807070652



MLA (The Modern Language Association) Style

Noura Hagaggi. "Production of an extracellular carboxymethyl cellulase from Exiguobacterium aurantiacum AN2 by submerged fermentation." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 14.1 (2018), -. Print. doi:10.5455/egyjebb.20170807070652



APA (American Psychological Association) Style

Noura Hagaggi (2018) Production of an extracellular carboxymethyl cellulase from Exiguobacterium aurantiacum AN2 by submerged fermentation. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 14 (1), -. doi:10.5455/egyjebb.20170807070652