E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


Optimization of peroxidase production from Humicola grisea and its application in biodegradation of textile dyes

Hany Moubasher, fatma mostafa, omima haroun.

Abstract
Peroxidases are important group of enzymes that play a major role in biodegradation of organopollutants. Eight fungal isolates were isolated, identified, and tested for their peroxidase production and decolorization activity. Sunzol Brilliant Orange and Suncron Blue were chosen for this investigation. All fungal isolates gave positive decolorization; however, the highest percentage of decolorization was obtained with Humicola grisea. Lignin peroxidases from Humicola grisea, which was the most potent fungus, was studied regarding the optimal culture conditions for production. The best lignin peroxidases (LiPs) production obtained using Kirk &Tien media supplemented with 10g l-1 sucrose as carbon source and 0.5g l-1 ammonium hydrogen phosphate as nitrogen source. The optimum temperature for LiPs production was at 30 °C, while the optimum pH was at 4.

Key words: Peroxidase, Humicola grisea, textile dyes, biodegradation, organopollutants.


 
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How to Cite this Article
Pubmed Style

Hany Moubasher, fatma mostafa, omima haroun. Optimization of peroxidase production from Humicola grisea and its application in biodegradation of textile dyes. Egypt. J. Exp. Biol. (Bot.). 2017; 13(1): 41-48. doi:10.5455/egyjebb.20170326105736


Web Style

Hany Moubasher, fatma mostafa, omima haroun. Optimization of peroxidase production from Humicola grisea and its application in biodegradation of textile dyes. https://www.egyseb.net//?mno=262040 [Access: December 03, 2023]. doi:10.5455/egyjebb.20170326105736


AMA (American Medical Association) Style

Hany Moubasher, fatma mostafa, omima haroun. Optimization of peroxidase production from Humicola grisea and its application in biodegradation of textile dyes. Egypt. J. Exp. Biol. (Bot.). 2017; 13(1): 41-48. doi:10.5455/egyjebb.20170326105736



Vancouver/ICMJE Style

Hany Moubasher, fatma mostafa, omima haroun. Optimization of peroxidase production from Humicola grisea and its application in biodegradation of textile dyes. Egypt. J. Exp. Biol. (Bot.). (2017), [cited December 03, 2023]; 13(1): 41-48. doi:10.5455/egyjebb.20170326105736



Harvard Style

Hany Moubasher, fatma mostafa, omima haroun (2017) Optimization of peroxidase production from Humicola grisea and its application in biodegradation of textile dyes. Egypt. J. Exp. Biol. (Bot.), 13 (1), 41-48. doi:10.5455/egyjebb.20170326105736



Turabian Style

Hany Moubasher, fatma mostafa, omima haroun. 2017. Optimization of peroxidase production from Humicola grisea and its application in biodegradation of textile dyes. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 13 (1), 41-48. doi:10.5455/egyjebb.20170326105736



Chicago Style

Hany Moubasher, fatma mostafa, omima haroun. "Optimization of peroxidase production from Humicola grisea and its application in biodegradation of textile dyes." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 13 (2017), 41-48. doi:10.5455/egyjebb.20170326105736



MLA (The Modern Language Association) Style

Hany Moubasher, fatma mostafa, omima haroun. "Optimization of peroxidase production from Humicola grisea and its application in biodegradation of textile dyes." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 13.1 (2017), 41-48. Print. doi:10.5455/egyjebb.20170326105736



APA (American Psychological Association) Style

Hany Moubasher, fatma mostafa, omima haroun (2017) Optimization of peroxidase production from Humicola grisea and its application in biodegradation of textile dyes. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 13 (1), 41-48. doi:10.5455/egyjebb.20170326105736