E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


EXPLORING THE ANTIMICROBIAL POTENTIAL OF LOCAL MARINE FUNGI

Dalia A.M. Abdou& 1608; Noura A. El-Far, Youssria M. Shetaia, Wael S.I. Abou-Elmagd, Al-Zahraa A. Karam El-Din.

Abstract
In search for bioactive compounds, 88 fungal isolates were collected from three Egyptian marine habitats. Crude extracts of 17 marine derived fungal isolates out of the 88 isolates showed variable activity against some human pathogenic bacterial strains (Salmonella enterica, Proteus sp., Klebseilla pneumonia, Staphylococcus aureus and Escherichia coli). The most potent fungal isolate that showed the highest antimicrobial activity was molecularly identified as Aspergillus welwetsichiae. Optimization of culture conditions of A. welwetsichiae revealed that the highest antimicrobial activity was obtained in shaking conditions (150rpm), using Czapek- dox medium, at pH 9 and incubation at 25C for 3 days. Salmonella enterica was the most sensitive test organism regarding the highest inhibition zone through all the optimization tests. Ethyl acetate extract of A. welwetsichiae was subjected to chemical analysis and declared that the active antibacterial metabolite is a mixture of acid (Mono (2-ethylhexyl) phthalate) and ester (bis (2-ethylhexyl) phthalate). Retesting the two compounds separately, they showed no activity which confirmed the synergistic antibacterial effect of the two compounds together. Upon testing the cytotoxic effect of the active antibacterial metabolite (mixture of acid and ester) against human lung fibroblast normal cell (WI-38 cell line), the results showed that there was a very weak inhibitory activity suggesting the possibility of considering it as a safe compound for further steps as a pharmaceutical product.

Key words: Marine derived fungi, Antimicrobial compounds, Aspergillus


 
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How to Cite this Article
Pubmed Style

DAAa, . EXPLORING THE ANTIMICROBIAL POTENTIAL OF LOCAL MARINE FUNGI. Egypt. J. Exp. Biol. (Bot.). 2016; 12(2): 275-288. doi:10.5455/egyjebb.20161221102915


Web Style

DAAa, . EXPLORING THE ANTIMICROBIAL POTENTIAL OF LOCAL MARINE FUNGI. http://www.egyseb.net//?mno=253782 [Access: November 03, 2020]. doi:10.5455/egyjebb.20161221102915


AMA (American Medical Association) Style

DAAa, . EXPLORING THE ANTIMICROBIAL POTENTIAL OF LOCAL MARINE FUNGI. Egypt. J. Exp. Biol. (Bot.). 2016; 12(2): 275-288. doi:10.5455/egyjebb.20161221102915



Vancouver/ICMJE Style

DAAa, . EXPLORING THE ANTIMICROBIAL POTENTIAL OF LOCAL MARINE FUNGI. Egypt. J. Exp. Biol. (Bot.). (2016), [cited November 03, 2020]; 12(2): 275-288. doi:10.5455/egyjebb.20161221102915



Harvard Style

, D. A. A. a. . & (2016) EXPLORING THE ANTIMICROBIAL POTENTIAL OF LOCAL MARINE FUNGI. Egypt. J. Exp. Biol. (Bot.), 12 (2), 275-288. doi:10.5455/egyjebb.20161221102915



Turabian Style

, Dalia A.M. Abdou and , and Noura A. El-Far, Youssria M. Shetaia, Wael S.I. Abou-Elmagd , Al-Zahraa A. Karam El-Din. 2016. EXPLORING THE ANTIMICROBIAL POTENTIAL OF LOCAL MARINE FUNGI. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 12 (2), 275-288. doi:10.5455/egyjebb.20161221102915



Chicago Style

, Dalia A.M. Abdou and , and Noura A. El-Far, Youssria M. Shetaia, Wael S.I. Abou-Elmagd , Al-Zahraa A. Karam El-Din. "EXPLORING THE ANTIMICROBIAL POTENTIAL OF LOCAL MARINE FUNGI." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 12 (2016), 275-288. doi:10.5455/egyjebb.20161221102915



MLA (The Modern Language Association) Style

, Dalia A.M. Abdou and , and Noura A. El-Far, Youssria M. Shetaia, Wael S.I. Abou-Elmagd , Al-Zahraa A. Karam El-Din. "EXPLORING THE ANTIMICROBIAL POTENTIAL OF LOCAL MARINE FUNGI." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 12.2 (2016), 275-288. Print. doi:10.5455/egyjebb.20161221102915



APA (American Psychological Association) Style

, D. A. A. a. . & (2016) EXPLORING THE ANTIMICROBIAL POTENTIAL OF LOCAL MARINE FUNGI. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 12 (2), 275-288. doi:10.5455/egyjebb.20161221102915