E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


Changes in tryptophan production level in maize callus through modification of (ASA2) gene for enhancing defense mechanism

Manal M. Abdel-Rahman, Nader E Mohamed, Ibrahim E Mousa.

Abstract
Anthranilate synthase (AS) is a key regulatory enzyme in tryptophan (Trp) biosynthesis and feedback inhibition by Trp. it is used as a biomarker of pathophysiological and physiological conditions mediated By ASA2 gene. In this study, we investigated the effect of tryptophan modifications by ASA2 gene and the possible importance of its modification in enhancing defense mechanism. The gene encoding ASA2α&β under the control of 35S promoter was introduced into maize callus Type II by particle bombardment with 1100 psi single and double shot. A total of eleven different transgenic lines showed markedly increased accumulation of free Trp. In spite of the marked increase in free Trp content, metabolic profiling was done by high – performance liquid chromatography coupled with amino acid analyzer. The results of this study pointed to develop a reproducible and efficient method for inserting genes into nuclear genome of many important plant species using the naturally occurring feedback-insensitive anthranilate synthase gene ASA2 from tobacco. The double shot protocol recorded low number of amino acids of produced plantlets and significant increases in Trp concentration comparing to single shot and control. These results indicate that transformation with ASA2α& β gene is a reliable approach to improve the defense mechanism of maize and increase the nutritional quality of maize which is very important for human and animal food.

Key words: Maize, Defense Mechanism, tryptophan, anthranilate synthase gene


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Manal M. Abdel-Rahman
Articles by Nader E Mohamed
Articles by Ibrahim E Mousa
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Manal M. Abdel-Rahman, Nader E Mohamed, Ibrahim E Mousa. Changes in tryptophan production level in maize callus through modification of (ASA2) gene for enhancing defense mechanism. Egypt. J. Exp. Biol. (Bot.). 2017; 13(2): 383-390. doi:10.5455/egyjebb.20170721075802


Web Style

Manal M. Abdel-Rahman, Nader E Mohamed, Ibrahim E Mousa. Changes in tryptophan production level in maize callus through modification of (ASA2) gene for enhancing defense mechanism. http://www.egyseb.net//?mno=250535 [Access: November 03, 2020]. doi:10.5455/egyjebb.20170721075802


AMA (American Medical Association) Style

Manal M. Abdel-Rahman, Nader E Mohamed, Ibrahim E Mousa. Changes in tryptophan production level in maize callus through modification of (ASA2) gene for enhancing defense mechanism. Egypt. J. Exp. Biol. (Bot.). 2017; 13(2): 383-390. doi:10.5455/egyjebb.20170721075802



Vancouver/ICMJE Style

Manal M. Abdel-Rahman, Nader E Mohamed, Ibrahim E Mousa. Changes in tryptophan production level in maize callus through modification of (ASA2) gene for enhancing defense mechanism. Egypt. J. Exp. Biol. (Bot.). (2017), [cited November 03, 2020]; 13(2): 383-390. doi:10.5455/egyjebb.20170721075802



Harvard Style

Manal M. Abdel-Rahman, Nader E Mohamed, Ibrahim E Mousa (2017) Changes in tryptophan production level in maize callus through modification of (ASA2) gene for enhancing defense mechanism. Egypt. J. Exp. Biol. (Bot.), 13 (2), 383-390. doi:10.5455/egyjebb.20170721075802



Turabian Style

Manal M. Abdel-Rahman, Nader E Mohamed, Ibrahim E Mousa. 2017. Changes in tryptophan production level in maize callus through modification of (ASA2) gene for enhancing defense mechanism. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 13 (2), 383-390. doi:10.5455/egyjebb.20170721075802



Chicago Style

Manal M. Abdel-Rahman, Nader E Mohamed, Ibrahim E Mousa. "Changes in tryptophan production level in maize callus through modification of (ASA2) gene for enhancing defense mechanism." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 13 (2017), 383-390. doi:10.5455/egyjebb.20170721075802



MLA (The Modern Language Association) Style

Manal M. Abdel-Rahman, Nader E Mohamed, Ibrahim E Mousa. "Changes in tryptophan production level in maize callus through modification of (ASA2) gene for enhancing defense mechanism." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 13.2 (2017), 383-390. Print. doi:10.5455/egyjebb.20170721075802



APA (American Psychological Association) Style

Manal M. Abdel-Rahman, Nader E Mohamed, Ibrahim E Mousa (2017) Changes in tryptophan production level in maize callus through modification of (ASA2) gene for enhancing defense mechanism. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 13 (2), 383-390. doi:10.5455/egyjebb.20170721075802