E-ISSN 2090-0503 | ISSN 1687-7497
 
2023, Vol: 19, Issue: 2
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Original Research 


REGENERATION OF HORSERADISH TREE (MORINGA OLEIFERA LAM.) THROUGH SOMATIC EMBRYOGENESIS AND SUSPENSION CULTURE

Gihan M. H. Hussein Hanan F. M. Shaaban.

Abstract
Using leaves or cotyledon as explants, we have developed a method for somatic embryogenesis in Moringa oleifera. Sections about 0.5 cm in length were cultured on MS medium supplemented with 2,4-D or TDZ for inducing embryonic calli. The best embryogenic calli produced on media Hg5 and Hg7 containing 3 and 5 TDZ mg/l, respectively, with 30-gram sucrose. Callus was then transferred to differentiation medium supplemented with BA, NAA and/or ABA. Rooting occurred on medium containing 40 µg/l NAA. Cell suspension culture was initiated from leaf-derived callus on MS liquid medium supplemented with 3 mg/l TDZ. Callus showed globular and torpedo stages after 4-6 weeks incubation. This regeneration method is expected to facilitate the development of a more efficient transformation system for Moringa oleifera. In our study, we also estimated phenolic compounds and flavonoid content in seeds, leaves, and contyledon and leaf-derived callus. The highest content of phenolic compounds was observed in seeds but flavonoids highest content was observed in leaves followed by leaf derived-callus. The production of callus and cell suspension cultures in the present study can be also used in the future in the mass production of Moringa oleifera secondary compounds.

Key words: Moringa oleifera, callus, somatic embryogenesis, cell suspension, flavonoids, phenolic compounds.


 
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Pubmed Style

Gihan M. H. Hussein Hanan F. M. Shaaban. REGENERATION OF HORSERADISH TREE (MORINGA OLEIFERA LAM.) THROUGH SOMATIC EMBRYOGENESIS AND SUSPENSION CULTURE. Egypt. J. Exp. Biol. (Bot.). 2016; 12(1): 89-96.

Web Style

Gihan M. H. Hussein Hanan F. M. Shaaban. REGENERATION OF HORSERADISH TREE (MORINGA OLEIFERA LAM.) THROUGH SOMATIC EMBRYOGENESIS AND SUSPENSION CULTURE. https://www.egyseb.net//?mno=228453 [Access: December 05, 2023].

AMA (American Medical Association) Style

Gihan M. H. Hussein Hanan F. M. Shaaban. REGENERATION OF HORSERADISH TREE (MORINGA OLEIFERA LAM.) THROUGH SOMATIC EMBRYOGENESIS AND SUSPENSION CULTURE. Egypt. J. Exp. Biol. (Bot.). 2016; 12(1): 89-96.


Vancouver/ICMJE Style

Gihan M. H. Hussein Hanan F. M. Shaaban. REGENERATION OF HORSERADISH TREE (MORINGA OLEIFERA LAM.) THROUGH SOMATIC EMBRYOGENESIS AND SUSPENSION CULTURE. Egypt. J. Exp. Biol. (Bot.). (2016), [cited December 05, 2023]; 12(1): 89-96.


Harvard Style

Gihan M. H. Hussein Hanan F. M. Shaaban (2016) REGENERATION OF HORSERADISH TREE (MORINGA OLEIFERA LAM.) THROUGH SOMATIC EMBRYOGENESIS AND SUSPENSION CULTURE. Egypt. J. Exp. Biol. (Bot.), 12 (1), 89-96.


Turabian Style

Gihan M. H. Hussein Hanan F. M. Shaaban. 2016. REGENERATION OF HORSERADISH TREE (MORINGA OLEIFERA LAM.) THROUGH SOMATIC EMBRYOGENESIS AND SUSPENSION CULTURE. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 12 (1), 89-96.


Chicago Style

Gihan M. H. Hussein Hanan F. M. Shaaban. "REGENERATION OF HORSERADISH TREE (MORINGA OLEIFERA LAM.) THROUGH SOMATIC EMBRYOGENESIS AND SUSPENSION CULTURE." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 12 (2016), 89-96.


MLA (The Modern Language Association) Style

Gihan M. H. Hussein Hanan F. M. Shaaban. "REGENERATION OF HORSERADISH TREE (MORINGA OLEIFERA LAM.) THROUGH SOMATIC EMBRYOGENESIS AND SUSPENSION CULTURE." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 12.1 (2016), 89-96. Print.


APA (American Psychological Association) Style

Gihan M. H. Hussein Hanan F. M. Shaaban (2016) REGENERATION OF HORSERADISH TREE (MORINGA OLEIFERA LAM.) THROUGH SOMATIC EMBRYOGENESIS AND SUSPENSION CULTURE. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 12 (1), 89-96.


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