E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


DNA FINGERPRINTING OF MERCURY-RESISTANT BACILLUS ISOLATED FROM THE INDUSTRIAL AREAS IN CAIRO, EGYPT

Youssef A. Mawgoud.

Abstract
Five mercury-resistant bacteria were successfully isolated from eighteen soil samples, collected from two of the Nile river bank districts, north and south Cairo where mercury is dumping from the surrounding industrial regions. Isolates were able to grow on basal medium supplemented with 100 ppm HgCl2.The acridine orange treatment revealed that the mercury-resistant gene is not plasmid-borne. The biochemical analysis affiliated the isolates to genus Bacillus. Pulsed-field gel electrophoresis of their DNA digested with AluI produced a characteristic genomic fingerprinting pattern with common conserved regions among them.

Key words: AluI digestion, Bacillus, Fingerprinting, Mercury, PFGE, RFLP


 
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Pubmed Style

Youssef A. Mawgoud. DNA FINGERPRINTING OF MERCURY-RESISTANT BACILLUS ISOLATED FROM THE INDUSTRIAL AREAS IN CAIRO, EGYPT. Egypt. J. Exp. Biol. (Bot.). 2015; 11(1): 59-62.


Web Style

Youssef A. Mawgoud. DNA FINGERPRINTING OF MERCURY-RESISTANT BACILLUS ISOLATED FROM THE INDUSTRIAL AREAS IN CAIRO, EGYPT. https://www.egyseb.net//?mno=192247 [Access: December 05, 2023].


AMA (American Medical Association) Style

Youssef A. Mawgoud. DNA FINGERPRINTING OF MERCURY-RESISTANT BACILLUS ISOLATED FROM THE INDUSTRIAL AREAS IN CAIRO, EGYPT. Egypt. J. Exp. Biol. (Bot.). 2015; 11(1): 59-62.



Vancouver/ICMJE Style

Youssef A. Mawgoud. DNA FINGERPRINTING OF MERCURY-RESISTANT BACILLUS ISOLATED FROM THE INDUSTRIAL AREAS IN CAIRO, EGYPT. Egypt. J. Exp. Biol. (Bot.). (2015), [cited December 05, 2023]; 11(1): 59-62.



Harvard Style

Youssef A. Mawgoud (2015) DNA FINGERPRINTING OF MERCURY-RESISTANT BACILLUS ISOLATED FROM THE INDUSTRIAL AREAS IN CAIRO, EGYPT. Egypt. J. Exp. Biol. (Bot.), 11 (1), 59-62.



Turabian Style

Youssef A. Mawgoud. 2015. DNA FINGERPRINTING OF MERCURY-RESISTANT BACILLUS ISOLATED FROM THE INDUSTRIAL AREAS IN CAIRO, EGYPT. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 11 (1), 59-62.



Chicago Style

Youssef A. Mawgoud. "DNA FINGERPRINTING OF MERCURY-RESISTANT BACILLUS ISOLATED FROM THE INDUSTRIAL AREAS IN CAIRO, EGYPT." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 11 (2015), 59-62.



MLA (The Modern Language Association) Style

Youssef A. Mawgoud. "DNA FINGERPRINTING OF MERCURY-RESISTANT BACILLUS ISOLATED FROM THE INDUSTRIAL AREAS IN CAIRO, EGYPT." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 11.1 (2015), 59-62. Print.



APA (American Psychological Association) Style

Youssef A. Mawgoud (2015) DNA FINGERPRINTING OF MERCURY-RESISTANT BACILLUS ISOLATED FROM THE INDUSTRIAL AREAS IN CAIRO, EGYPT. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 11 (1), 59-62.