E-ISSN 2090-0503 | ISSN 1687-7497
 
2023, Vol: 19, Issue: 2
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Original Research 


ANTIMICROBIAL ACTIVITY AND IMPACT OF PROPOLIS ON MOLECULAR NATURE AND PROTEINS PATTERN OF CANDIDA ALBICANS

Nehad MM. Gumgumjee.

Abstract
The antimicrobial activity of propolis against 9 species of gram –positive and gram –negative bacteria and 2 strains of Candida albicans were tested by the agar-well diffusion method. The propolis impacts a nature and molecular pattern of Candida albicans were evaluated by SDS–PAGE and RAPD–PCR techniques. Ethanolic extracts of propolis (EEP) exerted a significant inhibitory effect on the tested pathogenic bacteria and fungi, particularly at concentrations 50 and 100 mg/ml. The antibacterial activity of EEP was higher against Gram-positive bacteria than gram- negative bacteria. The maximum inhibition zones were recorded against Micrococcus sp and MRSA followed by Staphylococcus aureus, Candida albicans 2, Bacillus subtilis, Streptococcus sp, Escherichia coli and Candida albicans 1. The least activity was against recorded Pseudomonas aeruginosa. The ethanolic extract of propolis (EEP) has similar or greater inhibitory effect than the standard antibiotics tested. Molecular characterization of Candida albicans by RAPD analysis clearly showed the effects of propolis on gene expression.

Key words: Propolis, Pathogenic Bacteria, Candida albicans, RAPD-PCR amplification condition, SDS-PAGE of cell protein, Antibiotics.


 
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Pubmed Style

Nehad MM. Gumgumjee. ANTIMICROBIAL ACTIVITY AND IMPACT OF PROPOLIS ON MOLECULAR NATURE AND PROTEINS PATTERN OF CANDIDA ALBICANS. Egypt. J. Exp. Biol. (Bot.). 2009; 5(0): 221-227.

Web Style

Nehad MM. Gumgumjee. ANTIMICROBIAL ACTIVITY AND IMPACT OF PROPOLIS ON MOLECULAR NATURE AND PROTEINS PATTERN OF CANDIDA ALBICANS. https://www.egyseb.net//?mno=187354 [Access: December 08, 2023].

AMA (American Medical Association) Style

Nehad MM. Gumgumjee. ANTIMICROBIAL ACTIVITY AND IMPACT OF PROPOLIS ON MOLECULAR NATURE AND PROTEINS PATTERN OF CANDIDA ALBICANS. Egypt. J. Exp. Biol. (Bot.). 2009; 5(0): 221-227.


Vancouver/ICMJE Style

Nehad MM. Gumgumjee. ANTIMICROBIAL ACTIVITY AND IMPACT OF PROPOLIS ON MOLECULAR NATURE AND PROTEINS PATTERN OF CANDIDA ALBICANS. Egypt. J. Exp. Biol. (Bot.). (2009), [cited December 08, 2023]; 5(0): 221-227.


Harvard Style

Nehad MM. Gumgumjee (2009) ANTIMICROBIAL ACTIVITY AND IMPACT OF PROPOLIS ON MOLECULAR NATURE AND PROTEINS PATTERN OF CANDIDA ALBICANS. Egypt. J. Exp. Biol. (Bot.), 5 (0), 221-227.


Turabian Style

Nehad MM. Gumgumjee. 2009. ANTIMICROBIAL ACTIVITY AND IMPACT OF PROPOLIS ON MOLECULAR NATURE AND PROTEINS PATTERN OF CANDIDA ALBICANS. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 5 (0), 221-227.


Chicago Style

Nehad MM. Gumgumjee. "ANTIMICROBIAL ACTIVITY AND IMPACT OF PROPOLIS ON MOLECULAR NATURE AND PROTEINS PATTERN OF CANDIDA ALBICANS." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 5 (2009), 221-227.


MLA (The Modern Language Association) Style

Nehad MM. Gumgumjee. "ANTIMICROBIAL ACTIVITY AND IMPACT OF PROPOLIS ON MOLECULAR NATURE AND PROTEINS PATTERN OF CANDIDA ALBICANS." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 5.0 (2009), 221-227. Print.


APA (American Psychological Association) Style

Nehad MM. Gumgumjee (2009) ANTIMICROBIAL ACTIVITY AND IMPACT OF PROPOLIS ON MOLECULAR NATURE AND PROTEINS PATTERN OF CANDIDA ALBICANS. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 5 (0), 221-227.


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The Egyptian Journal of Experimental Biology (Botany) publishes original scientific research concerning botanical sciences (Morphology, Taxonomy, Gen ... Read more.



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