E-ISSN 2090-0503 | ISSN 1687-7497
 
2023, Vol: 19, Issue: 2
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Original Research 


SOME FACTORS AFFECTING CALLUS AND SOMATIC EMBRYOGENESIS IN ANTHER CULTURE OF SOLANUM MELONGENA L.

Kadhim Mohammad Ibrahim Ahmed Kh. Ali.

Abstract
In the present study, microspore developmental stages of Solanum melongena L. Ishtar F1 hybrid were determined cytologically. The size and appearance of flower buds and anthers were determined morphologically. Cold pretreatment was applied to flower buds for 24, 48, or 96 hrs in order to induce the androgenetic ability. High percentage of callus induced on untreated anthers grown on Pelletier To medium which supplemented with 4 mg/l Naphthalene acetic acid (NAA) and 1 mg/l (kin). Different levels of kin were used for the induction of organogenesis in callus cultures obtained from anthers of control and cold pretreated flower buds. Callus tissues initiated roots when 0.1 mg/l kin was supplemented to the Pelletier To medium. Containing 5 mg/l of 2, 4-dichlorophenoxy acetic acid (2,4-D) and of kin, C medium gave high percentages of callus (10%) and normal-looking embryos (3.3%) obtained on anthers incubated in continuous dark after transferring to R medium which supplemented with 0.1 mg/l kin.

Key words: Solanum melongena, anther culture, cold pretreatment, light or dark regimes


 
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Pubmed Style

Kadhim Mohammad Ibrahim Ahmed Kh. Ali. SOME FACTORS AFFECTING CALLUS AND SOMATIC EMBRYOGENESIS IN ANTHER CULTURE OF SOLANUM MELONGENA L.. Egypt. J. Exp. Biol. (Bot.). 2011; 7(1): 83-88.

Web Style

Kadhim Mohammad Ibrahim Ahmed Kh. Ali. SOME FACTORS AFFECTING CALLUS AND SOMATIC EMBRYOGENESIS IN ANTHER CULTURE OF SOLANUM MELONGENA L.. https://www.egyseb.net//?mno=186932 [Access: December 06, 2023].

AMA (American Medical Association) Style

Kadhim Mohammad Ibrahim Ahmed Kh. Ali. SOME FACTORS AFFECTING CALLUS AND SOMATIC EMBRYOGENESIS IN ANTHER CULTURE OF SOLANUM MELONGENA L.. Egypt. J. Exp. Biol. (Bot.). 2011; 7(1): 83-88.


Vancouver/ICMJE Style

Kadhim Mohammad Ibrahim Ahmed Kh. Ali. SOME FACTORS AFFECTING CALLUS AND SOMATIC EMBRYOGENESIS IN ANTHER CULTURE OF SOLANUM MELONGENA L.. Egypt. J. Exp. Biol. (Bot.). (2011), [cited December 06, 2023]; 7(1): 83-88.


Harvard Style

Kadhim Mohammad Ibrahim Ahmed Kh. Ali (2011) SOME FACTORS AFFECTING CALLUS AND SOMATIC EMBRYOGENESIS IN ANTHER CULTURE OF SOLANUM MELONGENA L.. Egypt. J. Exp. Biol. (Bot.), 7 (1), 83-88.


Turabian Style

Kadhim Mohammad Ibrahim Ahmed Kh. Ali. 2011. SOME FACTORS AFFECTING CALLUS AND SOMATIC EMBRYOGENESIS IN ANTHER CULTURE OF SOLANUM MELONGENA L.. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 7 (1), 83-88.


Chicago Style

Kadhim Mohammad Ibrahim Ahmed Kh. Ali. "SOME FACTORS AFFECTING CALLUS AND SOMATIC EMBRYOGENESIS IN ANTHER CULTURE OF SOLANUM MELONGENA L.." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 7 (2011), 83-88.


MLA (The Modern Language Association) Style

Kadhim Mohammad Ibrahim Ahmed Kh. Ali. "SOME FACTORS AFFECTING CALLUS AND SOMATIC EMBRYOGENESIS IN ANTHER CULTURE OF SOLANUM MELONGENA L.." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 7.1 (2011), 83-88. Print.


APA (American Psychological Association) Style

Kadhim Mohammad Ibrahim Ahmed Kh. Ali (2011) SOME FACTORS AFFECTING CALLUS AND SOMATIC EMBRYOGENESIS IN ANTHER CULTURE OF SOLANUM MELONGENA L.. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 7 (1), 83-88.


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