E-ISSN 2090-0503 | ISSN 1687-7497
 

Original Research 


Antimicrobial activity of Citrus limon essential oil against Escherichia coli in minced meat

AMANY REYAD.

Abstract
The efficacy of lemon oil as a food preservative against the well-known meat pathogen was investigated. Escherichia coli was detected and identified from 20 samples of frozen minced beef by using VITEK II automated system. The disc diffusion method was used to test the antibacterial activity of lemon essential oil diluted in ethanol 70% against E. coli on agar plates. The oil presented a significant antimicrobial activity against the tested bacterium; the minimum inhibitory concentration (MIC) was determined to be 0.25 %, lemon oil (0.25 %) had a measurable inhibitory effect when mixed with beef infected with 106 CFU/ml bacterial load. SEM images of the treated E. coli revealed membrane rupture, abnormal shaped cells, and cellular damage. The volatile profile of the oil revealed that it is mainly composed of monoterpenes, with citral, 6-exohydroxy camphene, and fenchone as the major compounds. The lemon essential oil could be a good alternative for existing biocides due to its potent antibacterial properties and its safety to the environment and human health.

Key words: E. coli, lemon oil, GC-MS, SEM, antimicrobial


 
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Pubmed Style

AMANY REYAD. Antimicrobial activity of Citrus limon essential oil against Escherichia coli in minced meat. Egypt. J. Exp. Biol. (Bot.). 2022; 18(2): 239-247. doi:10.5455/egyjebb.20220830025307


Web Style

AMANY REYAD. Antimicrobial activity of Citrus limon essential oil against Escherichia coli in minced meat. https://www.egyseb.net//?mno=110219 [Access: September 28, 2022]. doi:10.5455/egyjebb.20220830025307


AMA (American Medical Association) Style

AMANY REYAD. Antimicrobial activity of Citrus limon essential oil against Escherichia coli in minced meat. Egypt. J. Exp. Biol. (Bot.). 2022; 18(2): 239-247. doi:10.5455/egyjebb.20220830025307



Vancouver/ICMJE Style

AMANY REYAD. Antimicrobial activity of Citrus limon essential oil against Escherichia coli in minced meat. Egypt. J. Exp. Biol. (Bot.). (2022), [cited September 28, 2022]; 18(2): 239-247. doi:10.5455/egyjebb.20220830025307



Harvard Style

AMANY REYAD (2022) Antimicrobial activity of Citrus limon essential oil against Escherichia coli in minced meat. Egypt. J. Exp. Biol. (Bot.), 18 (2), 239-247. doi:10.5455/egyjebb.20220830025307



Turabian Style

AMANY REYAD. 2022. Antimicrobial activity of Citrus limon essential oil against Escherichia coli in minced meat. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 18 (2), 239-247. doi:10.5455/egyjebb.20220830025307



Chicago Style

AMANY REYAD. "Antimicrobial activity of Citrus limon essential oil against Escherichia coli in minced meat." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 18 (2022), 239-247. doi:10.5455/egyjebb.20220830025307



MLA (The Modern Language Association) Style

AMANY REYAD. "Antimicrobial activity of Citrus limon essential oil against Escherichia coli in minced meat." THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany) 18.2 (2022), 239-247. Print. doi:10.5455/egyjebb.20220830025307



APA (American Psychological Association) Style

AMANY REYAD (2022) Antimicrobial activity of Citrus limon essential oil against Escherichia coli in minced meat. THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany), 18 (2), 239-247. doi:10.5455/egyjebb.20220830025307